Sample Dropoff

  • Submit samples online. Information below on ordering chip type and assay.
  • Make sure sample names or numbers on tubes match the order names or sample ID numbers.
  • Supply samples in clearly marked 0.2 or 0.5 ml tubes
  • Bring your samples to ALS 3012. 
  • Put your samples in one of our submission boxes with a slip of paper containing your name, your PI's name, date and Order ID#. 
  • Samples go in our -20°C freezer by the door in one of the boxes marked Bioanalyzer. 
  • For timely processing, let us know your samples have arrived.
  • Samples run by a lab technician as time permits.  May be a day or two before results are available

Sample Information for Submission

  • 1µl of RNA or DNA is loaded on the bioanalyzer.
  • RNA samples are heat denatured before loading.
  • The Pico chip is much more sensitive to contaminants than the Nano chip.
  • Lower concentrations may not work as well depending on the broadness of the peaks and number of bands and how accurately the concentration was determined.

Select Chip Type

Chip type Sizing Range Quantitative Range Maximum Salt Concentrations Samples
per chip
Minimum Sample Volume
RNA Nano  
Total RNA: 25–500 ng/μl
mRNA 25–250 ng/μl
100 mM Tris
0.1 mM EDTA
or 125 mM NaCI
15 mM MgCI2
12 2-3 µl
RNA Pico   Total RNA: 50–5000 pg/μl  (in water)*
mRNA 250–5000 pg/μl   (in water)*
50 mM Tris
0.1 mM EDTA
or 125 mM NaCI
15 mM MgCI2
11 2-3 µl
HS-DNA  50-7000 bp 5 - 500 pg/µl  10 mM Tris
1 mM EDTA
11 2-3 µl

     *  Qualitative range

    Select Assay

    To select the correct assay for your sample type, see chart below. Consult with Anne-Marie Girard if you have questions about selection.

    Nucleic Acid Type Organism Type Description/Defining Factor Assay
    Total RNA    Eukaryotic  Only 18S, 28S bands, non-plant Eukaryotic Total RNA Assay
    Extra Ribosomal Bands, ie Plants Plant RNA Assay
    Prokaryotic  Extra Ribosomal Bands Plant RNA Assay
    Just 16S, 23S bands Prokaryotic Total RNA Assay
           
    mRNA Eukaryotic or 
    Prokaryotic
    Determine ribosomal contamination mRNA Assay
    Visualize only, shorter products <4000 Eukaryotic/Prokaryotic/Plant RNA assay
           
     cDNA or aRNA
      
    Eukaryotic or 
    Prokaryotic 
    Illumina library prep High Sensitivity DNA Assay
    Longer fragments >6000 for qPCR, use region feature in software mRNA Assay
    Shorter fragments <4000 for qPCR Eukaryotic Total RNA Assay
           
    Fragmented RNA
     
    Eukaryotic or 
    Prokaryotic  
    Just visualize size Eukaryotic Total RNA Assay
    Extract information from a region mRNA Assay
           
    DNA seq prep for HiSeq Eukaryotic or 
    Prokaryotic
    cDNA, amplicon, shotgun library, double stranded DNA 50-7000bp High Sensitivity DNA Assay

    Data is available for downloading online after samples have been run.