Keyence BZ-X710 Fluorescence Microscope

Objectives:

  • 2x/0.10 Plan Apo
  • 4x/0.13 Plan Fluor
  • 10x/0.3 Plan Fluor
  • 20x/0.45 Plan Fluor
  • 40x/0.6 Plan Fluor

Filter Cubes:

  • Dapi, GFP, Texas Red

Leica Stellaris 5 Confocal Microscope System

Details coming

ZEN Workstation

  • Z800 64bit 12GB RAM – DLD
  • Zen 2012 Desktop license
  • ZEN Deconvolution

Zeiss LSM 780 NLO Confocal Microscope System (RETIRED, unavailable)

  • Axio Observer.Z1
  • Inverted microscope
  • Motorized Scanning Stage
  • Triggering Hardware (user interface box)
  • Polarizers for anisotropy
  • X-Cite 120 PC
  • Point scanning confocal imaging
  • Multiphoton imaging
  • Lasers
    • Tunable Ti-Sapphire Coherent Chameleon Vision II laser
    • 405 nm Diode
    • Argon (458, 488, 514 nm)
    • 561 nm
    • 633 nm HeNe
  • Detectors
    • 32 channel spectral array GaAsP detector,
      • Also photon counting,
      • Fluorescence Correlation Spectroscopy (FCS)
    • plus two high sensitivity PMTs
    • BiG NDD (non-descanned) detectors
    • Transmitted light detector PMT
  • BiG filter sets Blue-Green and Green-Red
  • Incubation
    • Above room temperature and CO2 control for live cell timelapse
    • Y module and Temperature Module S: Cooling ( to below 0C) and heating (up to 90C?) two circulating bath incubation without CO2 control
  • Definite focus for NLO
  • Fluorescence Cubes Filter Sets
    • FL Filter Set 49 DAPI, Ex G365, FT 395, Ex BP 445/50
    • FL Filter Set 43 CY 3, Ex BP 545/25, FT 570, Em BP 605/70
    • FL Filter Set 38 HE GFP, Ex BP 470/40, FT 495, Em BP 525/50
    • FL Filter Set 50 Cy5, EX BP 640/30, FT 660, Em BP 690/50FL
    • Filter Set 46 YFP, Ex BP 500/20, FT 515, Em BP 535/30
    • FL Filter Set 47 CFP, Ex BP 436/20, FT 455,   Em BP 480/40
  • Objectives with DIC sliders
    • Plan-Apochromat 10x/0.45 WD=2.0 M27
    • Plan-Apochromat 20x/0.8 WD=0.55 M27
    • C-Apochromat 40x/1.2 W Korr. FCS
    • Plan-APO 40x/1.4 Oil DIC (UV) VIS-IR
    • Plan-Apochromat 63x/1.40 Oil DIC M27
  • Zen 2011 Software
    • FCS GAaSP
    • FRET Hardware
    • FRAP Analysis
    • ZEN 2011 Exp.Designer/MT/Tiles & Pos Pkg
    • ZEN Module 3D VisArt