The CQLS microscopes are open to users around campus. Anne-Marie Girard oversees the microscopes and can provide assisted imaging sessions, or training. Independent imaging time on the system can be reserved once training is completed.
Zeiss LSM 780 NLO Confocal Microscope System (ALS 2070)
An NSF Shared Instrumentation Grant supplemented by the OSU Research Office provided funding for the Zeiss LSM 780 NLO Confocal Microscope. The inverted microscope can generate either confocal images with the 32-channel internal GaAsP detector and two high sensitive internal PMTs, or multi-photon images with the internal detectors or to two GaAsP non-descanned detectors (NDD).
Axiovert 200 M Microscope (ALS 3131)
This is a widefield / epifluorescent microscope with an AxioCam MRm Rev.3 (firewire) camera and an X-Cite 120 LED System.
Microscope Analysis Workstation (ALS 2070)
An imaging analysis workstation is available for use with an extra copy of the ZEN software.
Other Microscopes Available at OSU
Keyence microscope (ALS 2070) Horticulture, contact Louisa Hooven
Zeiss PALM MicroBeam IV Laser Capture System, (SARL), contact Robyn Tanguay
Microscope Assisted Imaging & Training
Microscopy Registration Forms [PDF]
Arrange an assisted imaging session to look at your specimen. Please fill out the Microscopy Registration Form. Tell us as much as you can about your experiment in order to be the most helpful during your assisted session.
New users must complete training before using the system. Training consists of online material through CANVAS which covers background material, confocal usage policies, directions for imaging with our system, and quizzes on material. After you've finished the online work arrange hands-on training sessions, practice sessions and a basic practical test in order to use the system independently. Initial training sessions will be on a standard slide. Trainee must bring well-prepared samples to be imaged to the subsequent training sessions. Training sessions are geared toward individual samples. Basic training includes topics such as Z-stack, tile scan, time course. Advanced training is available on topics such as Multiphoton (Coherent Chameleon Vision II laser), Spectral Imaging, FRET, FRAP, incubation chamber use.
Axiovert 200M Microscope System Training
Training available for using the microscope with or without camera. Brightfield images, Fluorescent imaging, Multi-color images, Z-stacks and Timecourses.