Workstation

  • Z800 64bit 12GB RAM – DLD
  • Zen 2012 Desktop license
  • ZEN Deconvolution

Zeiss LSM 780 NLO Confocal Microscope System

  • Axio Observer.Z1
  • Inverted microscope
  • Motorized Scanning Stage
  • Triggering Hardware (user interface box)
  • Polarizers for anisotropy
  • X-Cite 120 PC
  • Point scanning confocal imaging
  • Multiphoton imaging
  • Lasers
    • Tunable Ti-Sapphire Coherent Chameleon Vision II laser
    • 405 nm Diode
    • Argon (458, 488, 514 nm)
    • 561 nm
    • 633 nm HeNe
  • Detectors
    • 32 channel spectral array GaAsP detector,
      • Also photon counting,
      • Fluorescence Correlation Spectroscopy (FCS)
    • plus two high sensitivity PMTs
    • BiG NDD (non-descanned) detectors
    • Transmitted light detector PMT
  • BiG filter sets Blue-Green and Green-Red
  • Incubation
    • Above room temperature and CO2 control for live cell timelapse
    • Y module and Temperature Module S: Cooling ( to below 0C) and heating (up to 90C?) two circulating bath incubation without CO2 control
  • Definite focus for NLO
  • Fluorescence Cubes Filter Sets
    • FL Filter Set 49 DAPI, Ex G365, FT 395, Ex BP 445/50
    • FL Filter Set 43 CY 3, Ex BP 545/25, FT 570, Em BP 605/70
    • FL Filter Set 38 HE GFP, Ex BP 470/40, FT 495, Em BP 525/50
    • FL Filter Set 50 Cy5, EX BP 640/30, FT 660, Em BP 690/50FL
    • Filter Set 46 YFP, Ex BP 500/20, FT 515, Em BP 535/30
    • FL Filter Set 47 CFP, Ex BP 436/20, FT 455,   Em BP 480/40
  • Objectives with DIC sliders
    • Plan-Apochromat 10x/0.45 WD=2.0 M27
    • Plan-Apochromat 20x/0.8 WD=0.55 M27
    • C-Apochromat 40x/1.2 W Korr. FCS
    • Plan-APO 40x/1.4 Oil DIC (UV) VIS-IR
    • Plan-Apochromat 63x/1.40 Oil DIC M27
  • Zen 2011 Software
    • FCS GAaSP
    • FRET Hardware
    • FRAP Analysis
    • ZEN 2011 Exp.Designer/MT/Tiles & Pos Pkg
    • ZEN Module 3D VisArt

Axiovert 200 M

  • Inverted microscope
  • Motorized Scanning Stage
  • X-Cite 120LED System
  • AxioCam MRm Rev.3 with FireWire
  • Axiovision SE64 Rel. 4.9.1 Software
  • Objectives
    • Dry Objectives
      • ACHROPLAN 4x/0,10 (44 00 20) (just oculars use)            
      • Plan-NEOFLUAR 10x/0,30 (44 03 30)
      • Plan-APOCHROMAT 20x/0,8 (440640-9903) 
      • Plan-NEOFLUAR 40x/0,75 (44 03 50)
    • Oil Immersion Objectives with DIC options
      • Plan-NEOFLUAR 40x/1.3 oil DIC (1056-602(1083-997))
      • Plan-APOCHROMAT 63x/1.4 oil DIC (1113-108)
    • Water Objectives
      • ACHROPLAN 40x/0,80W (44 00 90)
      • ACHROPLAN 63x/0,95W (1036-023)
    • Phase Objectives
      • N-Achroplan 10x/0.25 Ph 1 WD=6.5 M27
      • N-Achroplan 20x/0.45 Ph2 WD=0.63 M27
      • N-Achroplan 40x/0.65 Ph2 WD=0.60 M27
  • Fluorescence Cubes Filter Sets
    • FL Filter Set 02 Ex G365, FT395, Em LP420
    • FL Filter Set 10 Ex BP450-490, FT510, Em BP515-565
    • FL Filter Set 15 Ex BP546/12, FT580, Em LP590
    • FL Filter Set 50 Cy5, Ex BP640/30, FT 660, Em BP 690/50
    • FL Filter Set 46 YFP, Ex BP 500/20, FT 515, Em BP 535/30
    • FL Filter Set 47 CFP, Ex BP 436/20, FT 455, Em BP 480/40
  • Live Cell Incubation
    • Above room temperature and CO2 control physiological and perfusion chamber with objective heater

 

up