In June of 2021 the CQLS acquired the Illumina NextSeq 2000 sequencing instrument, as a replacement for the HiSeq 3000 (which will no longer be supported by Illumina in early 2023). More information about the NextSeq 2000 can be found on the specification sheet. There are three different sizes of flow cells available for the NextSeq 2000 that provide the following output:
P1- 100 million reads/run
P2- 400 million reads/run (300 million reads/run for 300bp PE)
P3- 1.2 billion reads/run
The following read lengths are available:
50bp single end (P3 only)
100bp single end
50bp paired end
100bp paired end
150bp paired end
300bp paired end (new starting 11/15/2022)
Please note, that unlike the HiSeq 3000 instrument, the flow cells for the NextSeq 2000 are not divided into separate lanes. The advantages of this are that there is no waiting for a run to start due to lanes not being filled, but all samples on a run must have unique barcodes/indices. Cost per base is cheaper with the higher throughput flow cells, so sharing runs will be an option. Please contact Mark Dasenko if you plan on sharing a run, as we will need to coordinate barcoding of samples.
The CQLS offers library prep and QC services prior to sequencing. To help the Illumina HTS user community, we have established an HTS mailing list where users can share information and announcements are posted by the Core Lab. Please contact Mark Dasenko with any questions regarding library prep and sequencing submission. Questions regarding the sequencing data output can be addressed by Matthew Peterson.