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CQLS has an older Qubit 1.0 available for use. (Manual) The Qubit quantitation platform is a simple, selective, and highly sensitive method for detecting DNA, RNA, or protein. As little as 1 uL of sample can be used. These assay kits and tubes from Invitrogen can be used with the Qubit. A few newer assays do not work with the Qubit 1.0 version such as the Qubit™ RNA IQ Assay. In addition, the Quant-iT kits contain fluorescent dyes that are specific to the biomolecule of interest and can also be used with the Qubit. See the Quant-iT assay protocol. You must schedule a time on the calendar in order to use the Qubit. Note: The CQLS Core Facilities does not supply tubes or assay kits for operating the Qubit.
Tubes:
Contact Anne-Marie Girard for any questions. Located in ALS 2156.
Cite this (Thermo Fisher Qubit fluorimeter, RRID:SCR_018095)