QubitCQLS has an older Qubit 1.0 available for use. (Manual) The Qubit quantitation platform is a simple, selective, and highly sensitive method for detecting DNA, RNA, or protein.  As little as 1 uL of sample can be used. These assay kits and tubes from Invitrogen can be used with the Qubit.  A few newer assays do not work with the Qubit 1.0 version such as the Qubit™ RNA IQ Assay. In addition, the Quant-iT kits contain fluorescent dyes that are specific to the biomolecule of interest and can also be used with the Qubit.  See the Quant-iT assay protocol. You must schedule a time on the calendar in order to use the Qubit.  Note: The CQLS Core Facilities does not supply tubes or assay kits for operating the Qubit.

Tubes:

  • 10011-830 Axygen PCR-05-C tubes (from VWR)
  • Q32856 Qubit assay tubes, 500 tubes

 

Contact Anne-Marie Girard  for any questions. Located in ALS 2156.

Cite this (Thermo Fisher Qubit fluorimeter, RRID:SCR_018095)