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The ABI PRISM® 7500 FAST Sequence Detection System is a fully integrated system for real-time detection of PCR. (Note: Currently HRM v3.2 also installed for High Resolution Melting experiments. Contact before use about calibration and mastermix). The system includes a built-in thermal cycler, a tungsten-halogen lamp to induce fluorescence, CCD (charge-coupled device) detector, real-time sequence detection software and high resolution melting software. Detection capabilities allow for SYBR, TaqMan and SYTO9 reagents applications. The 7500 FAST can process qPCR samples in 40 minutes (not including dissociation curve) with the appropriate chemistry.
Location: ALS 3139
Calendar: Real-Time PCR. Reserve time for all use. Please sign up to optimize runs per day
Training: Needed before use.
Contact: For calendar access, training or after hours access.
Software: 7500 FAST System SDS v 2.3, Set up templates, Analysis. (and v 3.2 High Resolution Melting (HRM) software. Contact if interested). Review release notes for compatibility.
Cite Instrument: Applied Biosystems 7500 Real-Time PCR System, RRID:SCR_018051
Resources:
High Resolution Melting Guide (Note, currently not installed, contact if interested)
Minimum Information about a qPCR Experiment guidelines and MIQE: Minimum Information for Publication of Quantitative Real-Time PCR Experiments
Consumables: Compatible plates and tubes for the 7500 FAST block