The ABI PRISM® 7500 FAST Sequence Detection System is a fully integrated system for real-time detection of PCR. (Note: Currently HRM v3.2 also installed for High Resolution Melting experiments. Contact before use about calibration and mastermix).  The system includes a built-in thermal cycler, a tungsten-halogen lamp to induce fluorescence, CCD (charge-coupled device) detector, real-time sequence detection software and high resolution melting software.  Detection capabilities allow for SYBR, TaqMan and SYTO9 reagents applications. The 7500 FAST can process qPCR samples in 40 minutes (not including dissociation curve) with the appropriate chemistry.

Warning: Antivirus Software WARNING for 7500 Fast Real-Time PCR Users
  • The laptop attached to the 7500 FAST does NOT have antivirus software because it interferes with data collection.
  • It is YOUR responsibility to verify that any USB device (thumb/flash drive, hard drive, etc.) that you plug into this laptop is free of viruses and malware.
  • If you have any questions or believe your USB device has been infected by the laptop, immediately contact the Core Lab personnel in ALS 3012 or Matthew Peterson (7-3897)
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Location: ALS 3139

Calendar: Real-Time PCR. Reserve time for all use. Please sign up to optimize runs per day

Training: Needed before use.

Contact:  For calendar access, training or after hours access.

Software: 7500 FAST System SDS v 2.3, Set up templates, Analysis.  (and  v 3.2 High Resolution Melting (HRM) software. Contact if interested). Review release notes for compatibility.

Cite Instrument: Applied Biosystems 7500 Real-Time PCR System, RRID:SCR_018051


Consumables: Compatible plates and tubes for the 7500 FAST block

  • The investigators are responsible for purchasing all consumables.
  • Plates AB: part number 4346906
  • 8-strip tubes: part number 4358293
  • Caps for 8-strip tubes: part number 4323032