effective July 1, 2022

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For a full explanation of discounted external rates (DER), click here.

Service Service Type Title Fee Description Internal Feesort ascending Internal ID External Fee External ID DER Rate Per Item
Extraction Plant/Fungi Plant/Fungi DNA extraction (full plate = 96 samples)

Users of this service will provide sample tissue in appropriate microtube collection plates for use with extraction robotics. A full plate of samples (exactly 96 samples) required. Price includes supplies, reagents, and labor.

 $4.20 14115-04 $6.40 14128-04 $4.80 sample
NextSeq Sequencing P2_300bp-PE w/Kit

Fee includes costs of consumables, backup tape, service contract, labor, and illumina kits.

 $4,315.00 16234-11 $6,472.00 16236-11 $4,854.00 Run
PacBio Library Prep/QC PacBio Sequel II Iso-seq cDNA Generation

Generate cDNA for Iso-seq template preparation.

 $382.00 15091-02 $572.00 15092-02 $429.00 sample
Sanger Seq Cycle Sequencing Rxn Included Standard full plate Sanger sequencing (95)

Standard Sanger Sequencing of samples submitted in a plate as a set of 95 samples (full plate). Standard chemistry reaction, Cleanup, and Run. Full plate cost is the same whether 95 samples or less (down to 48) are submitted

 $376.00 11147-06 $564.00 11146-06 $423.00 plate
NextSeq Library Prep/QC PrepX Robotic RNA PolyA Enrichment

PolyA Enrichment of total RNA samples using the PrepX robot.

 $36.00 11412-17 $41.00 11214-17 $36.90 sample
MiSeq Library Prep/QC PrepX Robotic RNA PolyA Enrichment

PolyA Enrichment of total RNA samples using the PrepX robot.

 $36.00 11412-17 $41.00 11214-17 $36.90 sample
Biocomputing Tech Support Weekly tech support on High Performance Server, including GPGPU Servers

Weekly usage of high performance server with greater than 80 cores and greater than 1TB of RAM/memory or with a General Purpose GPU (GPGPU) with greater then 16GB memory per card. Users pay for sole access to a high performance server with this configuration and all software compiled and working. Fee includes software compile time, consultation, maintainence and management of scientific processing server time. Week starts on Sunday at 12:00am and ends on Saturday at 11:59pm. User support time M-F, 8am-5pm.

 $304.00 11478-22 $912.00 11232-07 $342.00 weekly per machine
Microscopy Hourly Confocal Hourly Usage

Peak time (8:30am - 5:30pm). A one hour minimum charge and any additional usage will be charged in 15 minutes increments. (technician on call)

 $30.00 9207-03 $45.00 11241-03 $33.75 hour
MiSeq Library Prep/QC Illumina Pooling

Dilution and pooling of Illumina libraries.

 $3.90 11412-11 $5.80 11214-11 $5.22 sample
NextSeq Library Prep/QC Illumina Pooling

Dilution and pooling of Illumina libraries.

 $3.90 11412-11 $5.80 11214-11 $5.22 sample
Sanger Seq Economy (no cycle sequencing) Economy Sanger Sequencing

Economy Sanger sequencing. Cleanup and run only. Minimum 35 samples for processing. If less than 35 samples submitted the order may be delayed one business day for more samples to be submitted for processing.

 $3.50 11147-07 $5.20 11146-07 $3.90 sample
MiSeq Library Prep/QC 16s Normalization & Pooling-1-47 Samples

Illumina 16s library normalization and pooling for sequencing. 1-47 samples processed at a time.

 $3.20 11412-07 $4.70 11214-07 $3.53 Sample
MiSeq Library Prep/QC 16s Amplicon PCR Cleanup-48-96 Samples

Illumina 16s amplicon AMPure XP magnetic bead cleanup. 48-96 samples processed at a time.

 $3.00 11412-04 $4.50 11214-04 $3.38 Sample
NextSeq Sequencing P3_100bp-SE w/ Kit

Fee includes costs of consumables, backup tape, service contract, labor, and illumina kits.

 $3,992.00 16234-07 $5,988.00 16236-07 $4,491.00 Run
NextSeq Sequencing P2_150bp-PE w/ Kit

Fee includes costs of consumables, backup tape, service contract, labor, and illumina kits.

 $3,977.00 16234-04 $5,965.00 16236-04 $4,473.75 Run
NextSeq Sequencing P2_100bp-PE w/ Kit

Fee includes costs of consumables, backup tape, service contract, labor, and illumina kits.

 $3,061.00 16234-02 $4,591.00 16236-02 $3,443.25 Run
Biocomputing Tech Support Machine Technical Support (PI owns unit, 25% usage)

75% shared machine maintenance, 8am-5pm.

 $28.00 11478-08 not offered monthly per machine
Biocomputing Storage Data storage (file server or ZFS) (PI doesn't own unit, 100% usage)

File storage requires much more customization, consulting & support than web/database server or processing server. Consult, maintain and manage scientific file server. M-F, 8am-5pm. LIMITED TO 6 MONTHS PER PROJECT PER GROUP and a maximum of 12 TB, projects with a continuing need for ZFS storage beyond 6 months are advised to purchase dedicated storage.

 $271.00 11478-13 $407.00 11232-05 $305.25 monthly per server
Extraction Plant/Fungi Lysis step (microtubes - plates of 96)

If CGRB Core provides microtube collection plates for use with CGRB Core specific extraction equipment.

 $24.20 14115-01 $36.30 14128-01 $27.23 plate
DDPCR ADG-Reader ddPCR ADG plus droplet reader charge - 1 Column

Sample charges per set of 8 samples to create droplets on the Automated Droplet Generator, then to read them on Droplet Reader. To be used along with appropriate setup charge

 $23.00 14524-03 $34.00 14545-03 $25.50 column

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