Library Prep

Illumina Stranded Total RNA Prep with Ribo-Zero Plus or Ribo-Zero Plus Microbiome

IIllumina DNA Library Prep

Illumina 16s/ITS amplicon library prep following the Earth Microbiome Project protocol. Each reaction run in triplicate, then pooled. 48-96 samples processed at a time.

Illumina 16s/ITS amplicon library prep following the Earth Microbiome Project protocol. Each reaction run in triplicate, then pooled. 1-47 samples processed at a time.

Illumina initial PCR targetting V3 and V4 regions. 48-96 samples processed at a time.

Illumina initial PCR targetting V3 and V4 regions. 1-47 samples processed at a time.

Illumina amplicon AMPure XP magnetic bead cleanup. 48-96 samples processed at a time.

Illumina amplicon AMPure XP magnetic bead cleanup. 1-47 samples processed at a time.

Illumina index PCR that adds the dual barcodes and sequencing adapters. 48-96 samples processed at a time.

Illumina index PCR that adds the dual barcodes and sequencing adapters. 1-47 samples processed at a time.