The CQLS Illumina NGS sequencing service operates two instruments: NextSeq2000 and MiSeq. The CQLS offers library prep and QC services prior to sequencing. To help the Illumina HTS user community, we have established an HTS mailing list where users can share information and announcements are posted by the Core Lab. Please contact Mark Dasenko with any questions regarding library prep and sequencing submission. Questions regarding the sequencing data output can be addressed by Justin Elser for the NextSeq 2000 or Steven Carrell for the MiSeq.
Illumina NextSeq2000
More information about the NextSeq 2000 can be found on the specification sheet. There are four different sizes of flow cells available for the NextSeq 2000 that provide the following output:
- P4- 1.8 billion paired reads/run
- P3- 1.2 billion paired reads/run
- P2- 400 million paired reads/run
- P1- 100 million paired reads/run
The following read lengths are available:
| Run Type | Run Configuration |
|---|---|
| P4 300 cycle | Paired End 2x150 bp |
| P4 200 cycle | Paired End 2x100 bp |
| P4 100 cycle | Single End 100 bp or Paired End 2x50 bp |
| P4 50 cycle | Single End 50 bp or Paired End 2x25 bp |
| P3 300 cycle | Paired End 2x150 bp |
| P3 200 cycle | Paired End 2x100bp |
| P3 100 cycle | Single End 100 bp or Paired End 2x50 bp |
| P3 50 cycle | Single End 50 bp or Paired End 2x25 bp |
| P2 600 cycle | Paired End 2x300 bp |
| P2 300 cycle | Paired End 2x150 bp |
| P2 200 cycle | Paired End 2x100 bp |
| P2 100 cycle | Single End 100 bp or Paired End 2x50 bp |
| P1 600 cycle | Paired End 2x300 bp |
| P1 300 cycle | Paired End 2x150 bp |
| P1 100 cycle | Single End 100 bp or Paired End 2x50 bp |
Submit a NextSeq 2000 sequencing request in RELMS.
Illumina MiSeq
More information about the MiSeq can be found on the specification sheet. There are four different sizes of flow cells available for the MiSeq that provide the following output estimates:
- v.3 - 25 million paired reads/run (with low diversity amplicon libraries, output will be similar to v.2)
- v.2 - 15 million paired reads/run
- v.2 Micro - 4 million paired reads/run
- v.2 Nano - 1 million paired reads/run
The following read lengths are available:
| Run Type | Run Configuration |
|---|---|
| v.3 600 cycle | Paired End 2x300 bp |
| v.3 150 cycle | Single End 150 bp or Paired End 2x75 bp |
| v.2 500 cycle | Paired End 2x250 bp |
| v.2 300 cycle | Paired End 2x150 bp |
| v.2 50 cycle | Single End 50 bp or Paired End 2x25 bp |
| v.2 Micro, 300 cycle | Paired End 2x150 bp |
| v.2 Nano, 500 cycle | Paired End 2x250 bp |
| v.2 Nano, 300 cycle | Paired End 2x150 bp |
Submit a MiSeq sequencing request in RELMS