The CQLS Illumina NGS sequencing service operates three instruments: NextSeq2000, MiSeq i100 and MiSeq. The CQLS offers library prep and QC services prior to sequencing. Please contact Mark Dasenko with any questions regarding library prep and sequencing submission. Questions regarding the sequencing data output can be addressed by Justin Elser.
Illumina NextSeq2000
More information about the NextSeq 2000 can be found on the specification sheet. There are four different sizes of flow cells available for the NextSeq 2000 that provide the following output:
- P4- 1.8 billion paired reads/run
- P3- 1.2 billion paired reads/run
- P2- 400 million paired reads/run
- P1- 100 million paired reads/run
The following read lengths are available:
| Run Type | Run Configuration |
|---|---|
| P4 300 cycle | Paired End 2x150 bp |
| P4 200 cycle | Paired End 2x100 bp |
| P4 100 cycle | Single End 100 bp or Paired End 2x50 bp |
| P4 50 cycle | Single End 50 bp or Paired End 2x25 bp |
| P3 300 cycle | Paired End 2x150 bp |
| P3 200 cycle | Paired End 2x100bp |
| P3 100 cycle | Single End 100 bp or Paired End 2x50 bp |
| P2 600 cycle | Paired End 2x300 bp |
| P2 300 cycle | Paired End 2x150 bp |
| P2 200 cycle | Paired End 2x100 bp |
| P2 100 cycle | Single End 100 bp or Paired End 2x50 bp |
| P1 600 cycle | Paired End 2x300 bp |
| P1 300 cycle | Paired End 2x150 bp |
| P1 100 cycle | Single End 100 bp or Paired End 2x50 bp |
Submit a NextSeq 2000 sequencing request in RELMS.
Illumina MiSeq i100
More information abou the MiSeq i100 can be found on the specification sheet. There are two different sizes of flow cells available for the MiSeq i100 that provide the following output:
- 25M - 25 million paired reads/run
- 5M - 5 million paired reads/run
The following read lengths are available:
| Run Type | Run Configuration |
|---|---|
| 25M 1000 cycle | Paired End 2x500 bp |
| 25M 600 cycle | Paired End 2x300 bp |
| 25M 300 cycle | Paired End 2x150 bp |
| 25M 100 cycle | Single End 100 bp or Paired End 2x50 bp |
| 5M 600 cycle | Paired End 2x300 bp |
| 5M 300 cycle | Paired End 2x150 bp |
Submit a MiSeq i100 sequencing request in RELMS.
Illumina MiSeq
More information about the MiSeq can be found on the specification sheet. There are four different sizes of flow cells available for the MiSeq that provide the following output estimates:
- v.3 - 25 million paired reads/run (with low diversity amplicon libraries, output will be similar to v.2)
- v.2 - 15 million paired reads/run
- v.2 Micro - 4 million paired reads/run
- v.2 Nano - 1 million paired reads/run
The following read lengths are available:
| Run Type | Run Configuration |
|---|---|
| v.3 600 cycle | Paired End 2x300 bp |
| v.3 150 cycle | Single End 150 bp or Paired End 2x75 bp |
| v.2 500 cycle | Paired End 2x250 bp |
| v.2 300 cycle | Paired End 2x150 bp |
| v.2 50 cycle | Single End 50 bp or Paired End 2x25 bp |
| v.2 Micro, 300 cycle | Paired End 2x150 bp |
| v.2 Nano, 500 cycle | Paired End 2x250 bp |
| v.2 Nano, 300 cycle | Paired End 2x150 bp |
Submit a MiSeq sequencing request in RELMS