Library Sample Submission

To submit a samples for sequencing, please submit an Illumina Sequencing Request in RELMS.
Please provide us with 30 uL (if possible) of 10 nM sample in Qiagen buffer EB (Tris-Cl 10mM, pH 8.5) supplemented with 0.1% Tween-20 (optional). Molar concentration sample calculation below:

Library concentration = 40ng/µL
Median size fragment = 170bp
Library molecular weight = 170bp × 650g/mol per bp = 110500g/mol
Molar concentration = 40ng/µL / 110500ng/nmol = 0.000362nmol/µL
= 0.362µM
= 362nM

We will perform a final quantification of your library by qPCR to get the most accurate concentration measurement.
We don't require libraries to have the sizing checked on the BioAnalyzer/TapeStation, but it is highly recommended. Sizing info is needed in order to get accurate molar concentrations for determining sequencing loading. If library sizing is not checked, then you much provide us with the median fragment sizing info.
We will spike-in a PhiX control library into all Illumina runs. Typically 1% spike-in for high base diversity libraries (eg. whole genome, RNAseq) and at least 10% for low base diversity libraries (eg. amplicon libraries).
All primary data and the data generated by the Illumina data analysis pipeline will be available for only 2 weeks on the CQLS file servers. Therefore, researchers will need to copy their data onto a file server they own or a collaborator's file server. External customers have the option of downloading their data via the web or providing an external USB hard drive. If you have questions about the computational requirements for downstream analysis of your data and/or access to file server space, please contact HPC Support.
Please drop off samples to the -20 freezer in Agricultural and Life Science (ALS) 3139. For off campus users, please mail samples frozen on ice to the following address:

Mark Dasenko
Center for Quantitative Life Sciences
Oregon State University
3012 Agricultural & Life Sciences Building
2750 SW Campus Way
Corvallis, OR 97331-8646