Library Prep Types
We offer several kinds of library prep for Illumina sequencing. These include:
- RNA-seq
- -Whole transciptome (mRNA enrichment or rRNA depletion)
- 3'-Taq-Seq (QuantSeq FWD and QuantSeq Pool)
- DNA-seq
- Whole genome (mechanical shearing or tagmentation)
- Targeted amplicon (16s, ITS, 18s, etc.)
- Custom library preps are also available upon request. Some examples include.
- small RNA
- ChIP-seq
- Methyl-seq
- Target Enrichment
Consult with us to determine the type of prep best for your experiment in addition to the quality and quantity requirements for samples. We accept libraries prepared by you as long as it fits into the parameters necessary for the Illumina sequencer.
Submit a library prep with sequencing request in RELMS
Hints For All Preps
Quality: A better quality sample will give a better library. gDNA should have absorbance ratio values A260/A280 of 1.8–2.0. Longer length genomic DNA is better and for some preps necessary. RNA quality should be checked with the bioanalyzer before library preps and is not included in the fee. RINs of 8-10 are best for most preps. If the RIN is less, talk to us about possible preps.
Quantity: We appreciate more DNA or RNA if you have sufficient material, especially if you have determined concentration by a spectrophotometer such as nanodrop rather than a fluorometric method such as Qubit. We will double check the concentration with the Qubit fluorometer before starting, then use the appropriate volume needed for the specific prep being used. Fluorometric quantification is included in the library prep fee.
Size Determination: After library prep is complete, the size distribution must be checked with the bioanalyzer HS-DNA chip or TapeStation HS DNA tape. This is not included in the prep fee.
Library Quantification: After library prep is complete, the concentrations will be checked by fluorometer, Qubit or fluorescent plate reader. This is included in the prep fee. A final concentration measurement by qPCR is required prior to sequencing on each library or the pool of libraries. This is not included in the prep fee.
Pooling: Library pooling is included in the library prep fees. Let us know how you would like your libraries pooled and in what ratios before we start the prep. Selection of barcodes for the pool is important to plan before library prep is started.